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Instructions For Use

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Read all instructions before use

RapidBac™ Vet has been validated in clinical studies for both dogs and cats, comparing RapidBac™ Vet results with gold-standard urine culture results.

Kit Components

  • Test strip
  • Test vial
  • Disposable pipette
  • Desiccant (to be discarded)

Precautions and Warnings

  • Do not open foil pouch prior to use
  • Observe applicable biohazard safety procedures when handling test strips and samples
  • Do not reuse any components of the test kit

Storage

  • Store at room temperature (10-30°C/50-86°F)
  • Do not use after expiration date on packaging

Urine Collection

  • Cystocentesis
  • Catheter
  • Clean catch

Step By Step Instructions

Step # 1

Using the disposable pipette, add five (5) drops of sample into the test vial.

Step # 2

Make sure all liquid is at the bottom of the test
vial by swirling it and tapping the test vial gently on a flat surface. Wait 5 minutes.

Step # 3

Swirl test vial gently again. This step is essential for proper detection.

Step # 4

Place test strip into test vial with arrows pointing down. Wait 10 minutes. Do not disturb the test strip or test vial during this time.

Step # 5

After 10 minutes, remove the test strip from the test vial and immediately interpret the results. Do not read the results after 15 minutes.

Step # 1

Using the disposable pipette, add five (5) drops of sample into the test vial.

1

Step # 2

Make sure all liquid is at the bottom of the test
vial by swirling it and tapping the test vial gently on a flat surface. Wait 5 minutes.

2

Step # 3

Swirl test vial gently again. This step is essential for proper detection.

3

Step # 4

Place test strip into test vial with arrows pointing down. Wait 10 minutes. Do not disturb the test strip or test vial during this time.

4

Step # 5

After 10 minutes, remove the test strip from the test vial and immediately interpret the results. Do not read the results after 15 minutes.

5

Interpreting the Results

Negative Result

Only Control Line (C) develops color; no bacteria detected.

Positive Result

Any color development at Line 1 or Line 2 indicates the presence of bacteria in the sample. Faint lines are valid results.

Interpretation:
If both Line 1 and Line 2 are present, a Gram-negative organism has been detected. Gram-negative bacterial species known to give this result include: E. coli, Klebsiella spp., Citrobacter spp., Enterobacter spp., and Serratia spp.

If Line 2 is present and Line 1 is absent, the detected organism may be Gram-positive or Gram-negative. Bacterial species known to give this result include: Proteus spp., Morganella spp., Providencia spp., Staphylococcus spp., Enterococcus spp., Actinomyces spp., and Actinobaculum spp.

In rare samples with high levels of Gram-negative bacteria, both Line 1 and Line 2 appear, but the Control Line is weak or absent – this result should be interpreted as positive Gram-negative organism detected.

Invalid Result

If Control Line does not develop color, the result is invalid – repeat test.

In rare cases, Line 1 appears but Line 2 does not, this is an invalid result.
Do not repeat the test. Contact us for assistance.

Negative Result

Only Control Line (C) develops color; no bacteria detected.

1

Positive Result

Any color development at Line 1 or Line 2 indicates the presence of bacteria in the sample. Faint lines are valid results.

Interpretation:
If both Line 1 and Line 2 are present, a Gram-negative organism has been detected. Gram-negative bacterial species known to give this result include: E. coli, Klebsiella spp., Citrobacter spp., Enterobacter spp., and Serratia spp.

If Line 2 is present and Line 1 is absent, the detected organism may be Gram-positive or Gram-negative. Bacterial species known to give this result include: Proteus spp., Morganella spp., Providencia spp., Staphylococcus spp., Enterococcus spp., Actinomyces spp., and Actinobaculum spp.

In rare samples with high levels of Gram-negative bacteria, both Line 1 and Line 2 appear, but the Control Line is weak or absent – this result should be interpreted as positive Gram-negative organism detected.

2

Invalid Result

If Control Line does not develop color, the result is invalid – repeat test.

In rare cases, Line 1 appears but Line 2 does not, this is an invalid result.
Do not repeat the test. Contact us for assistance.

3

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RapidBactmVet

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